Daniel H. Scharf團(tuán)隊(duì)正在招募博士后研究人員。

博士后崗位1：

項(xiàng)目背景 - 煙曲霉（Aspergillus fumigatus）是空氣中普遍存在的腐生真菌之一。人類(lèi)和動(dòng)物不斷大量吸入這種真菌的分生孢子。一般情況下，具有免疫能力的宿主會(huì)通過(guò)先天免疫機(jī)制將這些分生孢子清除。因此，A. fumigatus多年來(lái)被認(rèn)為是一種弱病原體。然而在這些宿主中可以觀察到兩種不常見(jiàn)的感染性臨床癥狀——曲霉瘤和過(guò)敏性支氣管肺曲霉病。隨著免疫抑制患者數(shù)量的增加，嚴(yán)重且通常是致命的曲霉病(目前世界上最常見(jiàn)的霉菌感染)的發(fā)生率急劇增加。我們實(shí)驗(yàn)室的研究重點(diǎn)包括（a）生物體的基因組和分子鑒定，（b）開(kāi)發(fā)可用于診斷曲霉菌病的臨床和實(shí)驗(yàn)室方法，（c）鑒定真菌在體內(nèi)發(fā)生感染過(guò)程中起作用的宿主和真菌因子，（d）鑒定新的抗真菌靶標(biāo)。

在這個(gè)項(xiàng)目中，應(yīng)聘者加入我們團(tuán)隊(duì)后將使用我們先進(jìn)的體外和離體模型系統(tǒng)來(lái)研究煙曲霉在致病過(guò)程中與宿主發(fā)生相互作用體系中的重要因子，重點(diǎn)研究煙曲霉與宿主上皮細(xì)胞的直接相互作用。總體目標(biāo)是闡明導(dǎo)致宿主受損的致病機(jī)制。

應(yīng)聘要求：應(yīng)聘者已獲得或即將獲得生命科學(xué)方向的博士學(xué)位，例如生物學(xué)，生物化學(xué)或微生物學(xué)等。此外，應(yīng)聘者應(yīng)具有良好的團(tuán)隊(duì)協(xié)作精神以及獨(dú)立開(kāi)展研究工作的能力。具有微生物學(xué)，分子生物學(xué)，感染生物學(xué)，細(xì)胞生物學(xué)，細(xì)胞培養(yǎng)，基因組學(xué)或真菌遺傳學(xué)等研究背景的優(yōu)先。

博士后崗位2：

項(xiàng)目背景-膠質(zhì)毒素Gliotoxin（GT）是ETP型真菌毒素的前體。 GT在煙曲霉的病理生物學(xué)中起關(guān)鍵作用。它能夠調(diào)節(jié)免疫反應(yīng)并誘導(dǎo)不同細(xì)胞類(lèi)型的細(xì)胞凋亡。它的毒性歸因于其分子內(nèi)的一個(gè)特殊的二硫鍵，這個(gè)二硫鍵也是所有ETP的功能基序。具體而言，GT誘導(dǎo)細(xì)胞凋亡，通過(guò)抑制蛋白酶體抑制NF-B活化并抑制血管生成。這里僅列舉GT對(duì)宿主細(xì)胞的幾點(diǎn)作用。由各種致病真菌產(chǎn)生的其他ETP也可能在這些真菌的毒力機(jī)制中起作用。

在這個(gè)項(xiàng)目中，應(yīng)聘者加入我們團(tuán)隊(duì)后將嘗試鑒定和分離新的ETP，并致力于闡明這些ETP各自的生物合成途徑。

應(yīng)聘要求：應(yīng)聘者已獲得或即將獲得博士學(xué)位，例如化學(xué)，生物學(xué)，生物化學(xué)或微生物學(xué)。此外，應(yīng)聘者應(yīng)具有良好的團(tuán)隊(duì)協(xié)作精神以及獨(dú)立開(kāi)展研究工作的能力。具有LC-MS，MS / MS，成像質(zhì)譜（IMS），HPLC，NMR，HRMS或LC-MS等操作經(jīng)驗(yàn)的應(yīng)聘者優(yōu)先。

博士后崗位3：

項(xiàng)目背景-膠質(zhì)毒素Gliotoxin（GT）是ETP型真菌毒素的前體。 GT在煙曲霉的病理生物學(xué)中起關(guān)鍵作用。它能夠調(diào)節(jié)免疫反應(yīng)并誘導(dǎo)不同細(xì)胞類(lèi)型的細(xì)胞凋亡。它的毒性歸因于其分子內(nèi)的一個(gè)特殊的二硫鍵，這個(gè)二硫鍵也是所有ETP的功能基序。具體而言，GT誘導(dǎo)細(xì)胞凋亡，通過(guò)抑制蛋白酶體抑制NF-B活化并抑制血管生成。這里僅列舉GT對(duì)宿主細(xì)胞的幾點(diǎn)作用。由各種致病真菌產(chǎn)生的其他ETP也可能在這些真菌的毒力機(jī)制中起作用。

該項(xiàng)目的目的是將天然產(chǎn)物生物合成途徑的知識(shí)運(yùn)用到有機(jī)化學(xué)方面。通過(guò)開(kāi)發(fā)新型化學(xué)酶合成工具實(shí)現(xiàn)。在許多途徑中，分子復(fù)雜性程度大部分是由酶的催化來(lái)實(shí)現(xiàn)的。根據(jù)這些酶在天然產(chǎn)物生物合成過(guò)程中是否能在關(guān)鍵步驟發(fā)揮生物催化劑的潛能，我們會(huì)進(jìn)一步研究這類(lèi)酶的特性。因此，我們將鑒定重要的酶并對(duì)其進(jìn)行結(jié)構(gòu)分析。

應(yīng)聘要求：應(yīng)聘者已獲得或即將獲得生命科學(xué)方向的博士學(xué)位，例如生物學(xué)，生物化學(xué)或微生物學(xué)等。此外，應(yīng)聘者應(yīng)具有良好的團(tuán)隊(duì)協(xié)作精神以及獨(dú)立開(kāi)展研究工作的能力。具有蛋白質(zhì)表達(dá)和純化，蛋白質(zhì)結(jié)晶，晶體處理和X射線數(shù)據(jù)收集，蛋白質(zhì)晶體學(xué)研究背景的應(yīng)聘者優(yōu)先。

基本要求

(1)優(yōu)秀博士研究生，畢業(yè)于中國(guó)“雙一流”大學(xué)或國(guó)外一流大學(xué)，年齡一般在35歲以下，外籍年齡放寬至40歲，身體健康。

(2)較強(qiáng)的科研創(chuàng)新能力和項(xiàng)目執(zhí)行能力。

(3)能夠全職從事博士后研究工作。

(4)以第一作者發(fā)表過(guò)SCI論文。

待遇：

1.按照浙江大學(xué)博士后管理的相關(guān)規(guī)定，薪酬待遇20-25萬(wàn)/年，并提供專(zhuān)業(yè)的科研指導(dǎo)和一流的科研環(huán)境。

2.博士后研究時(shí)間2-4年，由學(xué)校、導(dǎo)師、博士后本人根據(jù)研究項(xiàng)目確定。人事關(guān)系轉(zhuǎn)入浙江大學(xué)從事博士后研究工作3年以上的，可以申請(qǐng)高級(jí)專(zhuān)業(yè)技術(shù)職務(wù)。

3.博士后研究員在研究期間可申請(qǐng)各類(lèi)項(xiàng)目資助。

4.成果優(yōu)秀、學(xué)術(shù)成績(jī)突出的博士后研究人員，在出站時(shí)，可推薦其申請(qǐng)教師崗位。

所需材料：

應(yīng)聘者請(qǐng)將中英文個(gè)人簡(jiǎn)歷，以及已發(fā)表的文章，獲獎(jiǎng)證明等以壓縮包的形式發(fā)至dscharf1@arcor.de，郵件主題為application for postdoctoral research fellows+name+name of supervisor+liuxuehr.com。

聯(lián)系方式：

Dr. Daniel H. Scharf

E-mail: dscharf1@arcor.de

截止日期至招滿(mǎn)為止

Dr. Daniel H. Scharf’s group is recruiting postdoctoral researchers.

Position 1：

Project background – Aspergillus fumigatus is one of the most ubiquitous of the airborne saprophytic fungi. Humans and animals constantly inhale numerous conidia of this fungus. The conidia are normally eliminated in the immunocompetent host by innate immune mechanisms, and aspergilloma and allergic bronchopulmonary aspergillosis, uncommon clinical syndromes, are the only infections observed in such hosts. Thus, A. fumigatus was considered for years to be a weak pathogen. With increases in the number of immunosuppressed patients, however, there has been a dramatic increase in severe and usually fatal invasive aspergillosis, now the most common mold infection worldwide. The focus of our lab, include (a) genomic and molecular characterization of the organism, (b) clinical and laboratory methods available for the diagnosis of aspergillosis in immunocompetent and immunocompromised hosts, (c) identification of host and fungal factors that play a role in the establishment of the fungus in vivo, and (d) identification of novel antifungal targets.

In this project, the successful applicant will use our sophisticated in vitro and ex vivo model systems to investigate important aspects of Aspergillus fumigatus interaction networks during pathogenesis, focusing on the direct interaction of A. fumigatus with host epithelial cells. The overarching goal is to elucidate the pathogenicity mechanisms which lead to host damage.

Candidate’s profile – We expect a degree in Life Sciences (e.g. Biology, Biochemistry, or Microbiology). Furthermore, the applicant should be able to perform team-oriented as well as independent work. Practical experiences in one or more of the following subjects are beneficial: Microbiology, Molecular Biology, Infection Biology, Cell Biology. Practical experience in cell culture, genomics or fungal genetics is an advantage.

Position 2：

Project background –Gliotoxin (GT) is the prototype of the epidithiodioxopiperazine (ETP)-type fungal toxins. GT plays a critical role in the pathobiology of Aspergillus fumigatus. It modulates the immune response and induces apoptosis in different cell types. The toxicity has been attributed to the unusual intramolecular disulfide bridge, which is the functional motif of all ETPs. Specifically, GT induces apoptosis, prevents NF-B activation by inhibition of the proteasome and inhibits angiogenesis, to mention only some of its effects on host cells. Other ETPs produced by various pathogenic fungi might also play a role in the virulence mechanisms of these fungi.

In this project, the successful applicant will try to identify and isolate novel ETPs and work on the elucidation of their respective biosynthetic pathways.

Candidate’s profile – We expect a degree in Life Sciences (e.g. Chemistry, Biology, Biochemistry, or Microbiology). Furthermore, the applicant should be able to perform team-oriented as well as independent work. Practical experiences in one or more of the following subjects are beneficial: hands-on skills in LC-MS and MS/MS, imaging mass spectrometry (IMS), previous experience in purification (HPLC) and chemical characterization of diverse types of natural products by high field NMR, HRMS, LC-MS.

Position 3：

Position Project background –Gliotoxin (GT) is the prototype of the epidithiodioxopiperazine (ETP)-type fungal toxins. GT plays a critical role in the pathobiology of Aspergillus fumigatus. It modulates the immune response and induces apoptosis in different cell types. The toxicity has been attributed to the unusual intramolecular disulfide bridge, which is the functional motif of all ETPs. Specifically, GT induces apoptosis, prevents NF-B activation by inhibition of the proteasome and inhibits angiogenesis, to mention only some of its effects on host cells. Other ETPs produced by various pathogenic fungi might also play a role in the virulence mechanisms of these fungi.

The aim of the project is to translate the knowledge from biosynthetic pathways of natural products into organic chemistry, achieved by developing novel chemoenzymatic synthesis tools. In many pathways large parts of the molecular complexity are set up by tailoring enzymes. These enzymes will be studied regarding their potential to act as biocatalysts that can substitute late and crucial steps in total syntheses. Therefore, interesting candidate enzymes should be identified and structural characterized.

Candidate’s profile – We expect a degree in Life Sciences (e.g. Biology, Biochemistry, or Microbiology). Furthermore, the applicant should be able to perform team-oriented as well as independent work. Practical experiences in one or more of the following subjects are beneficial: protein expression, purification and characterization, hands-on experience in protein crystallization, crystal handling and X-ray data collection, experience in protein crystallography including data processing, structure determination and refinement is a plus.

Requirements

1. Basic requirements

The ideal candidates would be or possess:

（1） Outstanding newly PhD graduates from top overseas universities or universities listed in China’s “Double-First Class” initiative or preponderant disciplines, usually under 35 years old for Chinese applicant, 40 years old for Foreign applicant, and healthy.

（2） Strong capability of research innovation and project organization.

（3） Available for full-time post-doctoral research work.

2. Academic requirements

The candidates are supposed to meet the academic requirements of the School of Medicine, Zhejiang University

Package

1. The annual salary is around RMB200, 000 yuan, and the fringe benefits are subject to the relevant post-doctoral regulations of Zhejiang University.

2. The post-doctoral research lasts from 2 to 4 years determined by the school, supervisor, and postdoctoral fellow himself according to the research projects. Postdoctoral research fellows who have been in the position for 3 years or above may apply for senior professional and technical positions.

3. Postdoctoral research fellows can apply for various projects funding during their stay.

4. Postdoctoral research fellows with excellent performance and outstanding academic achievements can be recommended to apply for the position of teachers in the school when they leave the position.

Application materials

1. Resume

2. Academic achievements and rewards list

3. Supporting materials

Please submit the application materials to Dr. Daniel H. Scharf：dscharf1@arcor.de with the subject “application for postdoctoral research fellows+name+name of supervisor+liuxuehr.com”.

Contact information

Dr. Daniel H. Scharf

E-mail: dscharf1@arcor.de 

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