招聘簡介:

　　德國癌癥研究中心是德國最大的生物醫(yī)學研究機構。我們擁有約3000名員工，在癌癥研究領域開展了廣泛的科學項目。

　　信號轉導和生長控制的劃分正在尋求博士后（參考號：2019-0063）

　　多形性膠質母細胞瘤四級（GB）是人類最常見、最惡性的原發(fā)性腦腫瘤，其特點是增殖率高、侵襲性強、預后極差。盡管在這一領域進行了廣泛的研究，但標準的治療護理，包括最大限度的手術切除（如適用），加上放射治療和化療，多年來一直保持不變，等待著新的、更具針對性和更有效的治療方法的發(fā)展。

　　我們的部門有興趣了解膠質瘤細胞侵襲的分子機制，這是導致膠質母細胞瘤復發(fā)并最終導致患者死亡的主要原因之一。本課題旨在研究腫瘤微環(huán)境（TME）細胞與膠質瘤細胞之間的相互作用，以確定促進膠質瘤細胞侵襲的新分子途徑。為此，我們優(yōu)化了基于器官型腦切片培養(yǎng)（Eisemann et al.，2018，BMC Cancer 18:103）的體外侵入檢測，該方法允許操縱腫瘤細胞和微環(huán)境，從而在可靠的背景下研究細胞遷移的速率限制因素。

　　英文原文:

　　   The German Cancer Research Center is the largest biomedical research institution in Germany. With approximately 3,000 employees, we operate an extensive scientific program in the field of cancer research.

　　The Division of Signal Transduction and Growth Control is seeking a

　　Postdoc

　　(Ref-No. 2019-0063)

　　Glioblastoma multiforme WHO grade IV (GB) is the most common and most malignant primary brain tumor in humans characterized by a high proliferative rate, aggressive invasiveness and a very poor prognosis. Despite an extensive research in the field the standard care of treatment, consisting in maximal surgical resection when applicable, together with radio- and chemotherapy, has been unchanged for many years and awaits the development of novel, more targeted and effective therapies.

　　Our division is interested in understanding molecular mechanism underlying glioma cell invasion, which is one of the main causes of glioblastoma recurrence that eventually leads to patients death. The current project aims at investigating the cross-talk between cells of the tumor microenvironment (TME) and glioma cells in order to identify novel molecular pathways promoting glioma cell invasion. To this aim we optimized an ex vivo invasion assay based on organotypic brain slice culture (Eisemann et al., 2018, BMC Cancer 18:103), which allows manipulation of tumor cells as well as the microenvironment, permitting the investigation of rate limiting factors of cell migration in a reliable context.

　　Description:

　　The candidate will apply gain-of-function and loss-of-function approaches to define the role of candidate genes in tumor cell migration and invasion using state-of-the-art transgenic and CRISPR / gRNA knock-out technologies in glioma cell lines and tumor neurospheres inoculated into murine organotypic brain slices. These in vitro approaches will be complemented by in vivo studies based on orthotopic implantation of syngeneic glioma cell lines in mice. These cells newly generated in our laboratory, which we recently used to investigate the role of the transmembrane glycoprotein podoplanin in glioma progression (Eisemann et al., 2018, Neuro Oncol) constitute a highly valuable tool to study not only glioma cell invasion but different aspects of glioma cell biology.

　　The candidate will characterize the different newly syngeneic glioma cell lines via analysis of RNA and DNA sequencing combined with immunostaining of orthotopic gliomas and employ this preclinical mouse model to study the impact of the tumor microenvironment in brain tumor progression and therapy resistance.

　　Your profile:

　　We are looking for a highly motivated and ambitious postdoc with a master in the field of biology (or comparable degree) and a Ph.D. to complement our team. The candidate should have a strong background in molecular and cellular biology demonstrated by peer-reviewed publications and a strong interest in brain tumor biology. Excellent experimental and analytical skills in advanced cell culture techniques and mouse genetics including animal experimentation are mandatory. Familiarity with stereotactic device for brain cell implantation and experience with multi-omics datasets are a plus.

　　The position is limited to 2 years with the possibility of prolongation.

　　The position can in principle be part-time.

　　For further information please contact Prof. Dr. Peter Angel, phone +49 6221 42-4570.

　　The German Cancer Research Center is committed to increase the percentage of female scientists and encourages female applicants to apply. Among candidates of equal aptitude and qualifications, a person with disabilities will be given preference. To apply for a position please use our online application portal (">www.dkfz.de)*.

　　We ask for your understanding that we cannot return application documents that are sent to us by post (Deutsches Krebsforschungszentrum, Personalabteilung, Im Neuenheimer Feld 280, 69120 Heidelberg) and that we do not accept applications submitted via email. We apologize for any inconvenience this may cause.

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