丹麥哥本哈根大學(xué)分子生物學(xué)博士后職位
Job Description
Nano Science Center
Department of Chemistry
Faculty of Science
University of Copenhagen
University of Copenhagen, Department of Chemistry is offering a Postdoc position for up to three years from 1 June 2020 or soon as possible thereafter.
Background and description of the research
Our lab is developing disruptive technologies to study the biophysical properties of membranes and membrane proteins on the nanoscale using fluorescence microscopy. Membrane proteins are one of the most important classes of proteins in biology comprising more than 60% of existing pharmaceutical targets. Biological membranes enable the function of membrane proteins and therefore play a key role themselves in governing a plethora of biological processes. Our work uncovers hitherto unknown nanoscale properties of membrane and membrane proteins that ultimately dictate cell behaviour, and consequently provides a path towards new generations of therapeutic agents based on entirely new biological principles.
In a breakthrough paper in Science (Veshaguri et. al., 2016) we developed a method that resolved ionic currents with a million-fold higher sensitivity than the Nobel Prize awarded method of patch clamp (atto-amperes). This allowed us to observe for the first time the function of single transporters, revealing the existence of functional heterogeneity based on hitherto unknown off-cycle states (i.e. ultra-stable inactive and leaky states with lifetimes 105-fold longer than those of states in the classical transport cycle). The dramatic consequence of this unforeseen complexity is that many fundamental mechanistic tenets that were based on macroscopic experiments of transport will have to be revised.
Now we wish to further develop the assay described above to make it applicable to a wider range of transporters, in a more relevant biological context. Currently, the most widely applied method for single transporter study is the reconstitution of the transporter into artificial membranes, namely liposomes. Thus, a protein, often not expressed in an organism to which it is endogenous, is placed in a setting which doesn’t consist of its native lipid composition. To circumvent this, we aim to generate mammalian cell-derived vesicles occupied by transporters of interest. The task of the postdoc will be to spearhead our efforts in setting up a workflow which enables us to generate these vesicles, thus broadening the range of transporters avalible for single molecule study.
Requirements:
BSc and MSc in molecular biology
PhD focusing on extracellular vesicles
Experience in breast cancer, melanoma and colon cancer research
Experience working with laboratory animals
Experience in isolating extracellular vesicles from blood plasma
Experience in extracellular vesicle isolation from patient tissue interstitium
Cryosectioning of tissue
Immunohistochemistry
Cell culture experience
Experience handling and analyzing mass spectrometry data
Experience with Size Exclusion Chromatography
Experience performing differential, rate zonal and isopycnic ultracentrifugation
Experience with a FACS Aria and FlowJo software
Experience using a ZetaView nanoparticle tracking analyzer
Further information on the Department is linked at http://www.science.ku.dk/english/about-the-faculty/organisation/ Inquiries about the position can be made to Prof. Dimitrios Stamou, stamou@nano.ku.dk
The University wishes our staff to reflect the diversity of society and thus welcomes applications from all qualified candidates regardless of personal background.
Terms of employment
The position is covered by the Memorandum on Job Structure for Academic Staff.
Terms of appointment and payment accord to the agreement between the Ministry of Finance and The Danish Confederation of Professional Associations on Academics in the State.
The starting salary is currently up to DKK 434.179 including annual supplement (+ pension up to DKK 74.244). Negotiation for salary supplement is possible.
The application, in English, must be submitted electronically by clicking APPLY NOW below.
Please include
Curriculum vita
Diplomas (Master and PhD degree or equivalent)
Complete publication list
Separate reprints of 3 particularly relevant papers
The deadline for applications is March 16, 23:59 GMT +1.
After the expiry of the deadline for applications, the authorized recruitment manager selects applicants for assessment on the advice of the Interview Committee.
You can read about the recruitment process at http://employment.ku.dk/faculty/recruitment-process/
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