Post-Doctoral Fellow in Regulation of Mammalian Genome Expression M/F

Description

This project aims aims at understanding the role of secondary DNA structures and their ligands in the regulation of genome expression. He/She will perform genome editing and functional genomics experiments to support its investigations in the laboratory.

The post-doctoral fellow will join the IGMM, which is a plurithematic CNRS research unit, offering an environment perfectly suited to the development of the proposed subject. This unit located on the CNRS campus/ Route de Mende in Montpellier is part of a rich scientific environment composed by the various institutes (IRIM, CRBM, CEFE, etc). In addition, several technical platforms (flow cytometry, imaging, metabolism, etc.) are available and complete the site. The team Transcription and Epigenomics is interested in basal and activated transcription mechanisms as well as the regulation of gene expression through regulatory elements (promoters and enhancers). We are looking for a motivated and dynamic postdoc with good knowledge in genome editing and functional genomics. The selected applicant will be offered a 1rst one year that could be renewed for one or two extra years. More information on : https: // www. igmm.cnrs.fr/en/team/transcription- epigenomique-dans-les-cel... Recent publications of the group : Alternative Enhancer Usage and Targeted Polycomb Marking Hallmark Promoter Choice during T Cell Differentiation. Maqbool MA, Pioger L, El Aabidine AZ, Karasu N, Molitor AM, Dao LTM, Charbonnier G, van Laethem F, Fenouil R, Koch F, Lacaud G, Gut I, Gut M, Amigorena S, Joffre O, Sexton T, Spicuglia S, Andrau JC. Cell Rep. 2020 Aug 18;32(7):108048. doi: 10.1016/j.celrep.2020.108048. The Landscape of L1 Retrotransposons in the Human Genome Is Shaped by Pre- insertion Sequence Biases and Post-insertion Selection. Sultana T, van Essen D, Siol O, Bailly-Bechet M, Philippe C, Zine El Aabidine A, Pioger L, Nigumann P, Saccani S, Andrau JC, Gilbert N, Cristofari G. Mol Cell. 2019 May 2;74(3):555-570.e7. doi: 10.1016/j.molcel.2019.02.036. Epub 2019 Apr 4 Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals. Shah N, Maqbool MA, Yahia Y, El Aabidine AZ, Esnault C, Forné I, Decker TM, Martin D, Schüller R, Krebs S, Blum H, Imhof A, Eick D, Andrau JC. Mol Cell. 2018 Jan 4;69(1):48-61.e6. doi: 10.1016/j.molcel.2017.12.009. Dynamic recruitment of Ets1 to both nucleosome-occupied and -depleted enhancer regions mediates a transcriptional program switch during early T-cell differentiation. Cauchy P, Maqbool MA, Zacarias-Cabeza J, Vanhille L, Koch F, Fenouil R, Gut M, Gut I, Santana MA, Griffon A, Imbert J, Moraes-Cabé C, Bories JC, Ferrier P, Spicuglia S, Andrau JC. Nucleic Acids Res. 2016 May 5;44(8):3567-85. doi: 10.1093/nar/gkv1475. Epub 2015 Dec 15. Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells. Soucie EL, Weng Z, Geirsdóttir L, Molawi K, Maurizio J, Fenouil R, Mossadegh- Keller N, Gimenez G, VanHille L, Beniazza M, Favret J, Berruyer C, Perrin P, Hacohen N, Andrau JC, Ferrier P, Dubreuil P, Sidow A, Sieweke MH. Science. 2016 Feb 12;351(6274):aad5510. doi: 10.1126/science.aad5510. Epub 2016 Jan 21. High-throughput and quantitative assessment of enhancer activity in mammals by CapStarr-seq.

Alternative Enhancer Usage and Targeted Polycomb Marking Hallmark Promoter Choice during T Cell Differentiation. Maqbool MA, Pioger L, El Aabidine AZ, Karasu N, Molitor AM, Dao LTM, Charbonnier G, van Laethem F, Fenouil R, Koch F, Lacaud G, Gut I, Gut M, Amigorena S, Joffre O, Sexton T, Spicuglia S, Andrau JC. Cell Rep. 2020 Aug 18;32(7):108048. doi: 10.1016/j.celrep.2020.108048. The Landscape of L1 Retrotransposons in the Human Genome Is Shaped by Pre- insertion Sequence Biases and Post-insertion Selection. Sultana T, van Essen D, Siol O, Bailly-Bechet M, Philippe C, Zine El Aabidine A, Pioger L, Nigumann P, Saccani S, Andrau JC, Gilbert N, Cristofari G. Mol Cell. 2019 May 2;74(3):555-570.e7. doi: 10.1016/j.molcel.2019.02.036. Epub 2019 Apr 4 Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals. Shah N, Maqbool MA, Yahia Y, El Aabidine AZ, Esnault C, Forné I, Decker TM, Martin D, Schüller R, Krebs S, Blum H, Imhof A, Eick D, Andrau JC. Mol Cell. 2018 Jan 4;69(1):48-61.e6. doi: 10.1016/j.molcel.2017.12.009. Dynamic recruitment of Ets1 to both nucleosome-occupied and -depleted enhancer regions mediates a transcriptional program switch during early T-cell differentiation. Cauchy P, Maqbool MA, Zacarias-Cabeza J, Vanhille L, Koch F, Fenouil R, Gut M, Gut I, Santana MA, Griffon A, Imbert J, Moraes-Cabé C, Bories JC, Ferrier P, Spicuglia S, Andrau JC. Nucleic Acids Res. 2016 May 5;44(8):3567-85. doi: 10.1093/nar/gkv1475. Epub 2015 Dec 15. Lineage-specific enhancers activate self-renewal genes in macrophages and embryonic stem cells. Soucie EL, Weng Z, Geirsdóttir L, Molawi K, Maurizio J, Fenouil R, Mossadegh- Keller N, Gimenez G, VanHille L, Beniazza M, Favret J, Berruyer C, Perrin P, Hacohen N, Andrau JC, Ferrier P, Dubreuil P, Sidow A, Sieweke MH. Science. 2016 Feb 12;351(6274):aad5510. doi: 10.1126/science.aad5510. Epub 2016 Jan 21. High-throughput and quantitative assessment of enhancer activity in mammals by CapStarr-seq. Vanhille L, Griffon A, Maqbool MA, Zacarias-Cabeza J, Dao LT, Fernandez N, Ballester B, Andrau JC, Spicuglia S. Nat Commun. 2015 Apr 15;6:6905. doi: 10.1038/ncomms7905.

Eligibility criteria

The applicant should have a PhD in Genetics/Molecular Biology, preferably in the fields of transcription/chromatin and/or functional genomics. He/she will be familiar with CRISPR and high-throughput sequencing. Further knowledge in Bioinformatics analyses will be useful for the project but is not compulsory

Web site for additional job details

https: // emploi.cnrs.fr/Offres/CDD/UMR5535-SARADE-005/Default.aspx

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